Journal: Pharmaceutics

Article Title: Polydopamine-Coated Cu-BTC Nanowires for Effective Magnetic Resonance Imaging and Photothermal Therapy

doi: 10.3390/pharmaceutics15030822

Figure Lengend Snippet: ( a ) XRD analysis of NWs. ( b ) FTIR analysis of NWs.

Article Snippet: In addition, the functionality and qualitative and chemical compositions of the materials were evaluated using Fourier-transform infrared (FTIR) spectroscopy (Spotlight 200i Sp2 with AutoATR System, Perkin Elmer, Waltham, MA, USA) and X-ray photoelectron spectroscopy (XPS; JPS-9030, JEOL Ltd., Minato-ku, Japan).

Techniques:

Journal: Journal of Functional Foods

Article Title: Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma

doi: 10.1016/j.jff.2021.104781

Figure Lengend Snippet: Fig. 3. Cell cycle analysis using flow cytometry in A549 and H460 cells. A: Representative histogram of the G0/G1, S and G2/M phases of the gated cells for control, F4 (nTOPL) and CIS. B: The percentage of cell cycle distribution for A549 and H460 cells: At least 10,000 cells per sample were examined quantitatively for the distribution or proportion of the cells for each Sample. Data are expressed as mean ± standard deviation for triplicates within an individual experiment. Statistical significance was performed using one-way ANOVA. *p < 0.05 vs Control, #p < 0.05 vs CIS.

Article Snippet: Currently, there is an increasing optimism of nanomedicine as it Abbreviations: A549 cell line, The human squamous cell lung cancer cell line; AO, acridine orange; ATCC, American type culture collection; Bax, BCL2-Associated X Protein; Bcl2, B-cell lymphoma protein 2; Beclin-1, BECN1 gene encoded protein; involved in autophagy, apoptosis and host defense; C13 NMR, Carbon-13 nuclear magnetic resonance; CIS, Cisplatin (positive control); CT, Understanding cycle threshold; DDS, drug-delivery systems; Delta Ct, corresponds to the difference between CtSOI and Ct of reference sequence (RS); EE, Entrapment efficiency; FT-IR analysis, Fourier-transform infrared spectroscopy; G0/G1 cell cycle arrest, resting phase is a period in the cell cycle/the first growth period of the cell cycle during interphase; G2/M phase, The arrest in G2 is mediated by the inhibition of M-phase promoting factor (MPF); GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; GC–MS, Gas chromatography–mass spectrometry; GMS, glyceryl monostearate; H460 cell line, large cell lung cancer cell line; HPGPC, high-performance gel permeation chromatography; HPLC, high-performance liquid chromatography; Mn, The number average molecular weight; MTT assay, colorimetric assay for assessing cell metabolic activity; NFI, net fluorescent intensities; NSCLC, non-small lung cancer; nTOPL, nano- Taraxacum officinale polysaccharide; one-way ANOVA, Analysis of variance; PDI,PI, polydispersity index; qRT-PCR, Real-Time Quantitative Reverse Transcription PCR; S-phase, synthesis phase of the cell cycle in which DNA is replicated; SLNs, solid lipid nanoparticles; TEM, Transmission electron microscopy; TOPL, Taraxacum officinale leaves derived polysaccharides; UV spectrum, Ultraviolet spectrum; WI-38 cell line, normal human lung cell line; ZP, zeta potential.

Techniques: Cell Cycle Assay, Flow Cytometry, Control, Standard Deviation

Journal: Journal of Functional Foods

Article Title: Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma

doi: 10.1016/j.jff.2021.104781

Figure Lengend Snippet: Fig. 4. An Annexin-V/PI stain for studying apoptosis by flow cytometry. A: Representative histogram of different cell populations; Viable, early apoptotic, late apoptotic and necrotic cells for control, F4 (nTOPL) and CIS. B: The percent of cell population in A549 and H460 cells. The cell death mode was measured using at least 10,000 cells per sample. Data are expressed as mean ± standard deviation for triplicates within an individual experiment. Statistical significance was performed using one-way ANOVA. *p < 0.05 vs Control, #p < 0.05 vs CIS.

Article Snippet: Currently, there is an increasing optimism of nanomedicine as it Abbreviations: A549 cell line, The human squamous cell lung cancer cell line; AO, acridine orange; ATCC, American type culture collection; Bax, BCL2-Associated X Protein; Bcl2, B-cell lymphoma protein 2; Beclin-1, BECN1 gene encoded protein; involved in autophagy, apoptosis and host defense; C13 NMR, Carbon-13 nuclear magnetic resonance; CIS, Cisplatin (positive control); CT, Understanding cycle threshold; DDS, drug-delivery systems; Delta Ct, corresponds to the difference between CtSOI and Ct of reference sequence (RS); EE, Entrapment efficiency; FT-IR analysis, Fourier-transform infrared spectroscopy; G0/G1 cell cycle arrest, resting phase is a period in the cell cycle/the first growth period of the cell cycle during interphase; G2/M phase, The arrest in G2 is mediated by the inhibition of M-phase promoting factor (MPF); GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; GC–MS, Gas chromatography–mass spectrometry; GMS, glyceryl monostearate; H460 cell line, large cell lung cancer cell line; HPGPC, high-performance gel permeation chromatography; HPLC, high-performance liquid chromatography; Mn, The number average molecular weight; MTT assay, colorimetric assay for assessing cell metabolic activity; NFI, net fluorescent intensities; NSCLC, non-small lung cancer; nTOPL, nano- Taraxacum officinale polysaccharide; one-way ANOVA, Analysis of variance; PDI,PI, polydispersity index; qRT-PCR, Real-Time Quantitative Reverse Transcription PCR; S-phase, synthesis phase of the cell cycle in which DNA is replicated; SLNs, solid lipid nanoparticles; TEM, Transmission electron microscopy; TOPL, Taraxacum officinale leaves derived polysaccharides; UV spectrum, Ultraviolet spectrum; WI-38 cell line, normal human lung cell line; ZP, zeta potential.

Techniques: Staining, Flow Cytometry, Control, Standard Deviation

Journal: Journal of Functional Foods

Article Title: Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma

doi: 10.1016/j.jff.2021.104781

Figure Lengend Snippet: Fig. 5. Evaluation of Bax and Bcl2 expression upon F4 (nTOPL) treatment in A549 and H460 cells. A: The folds of Bax expression. B: The folds of Bcl2 expression. Data are expressed as mean ± standard deviation for triplicates within an individual experiment. Statistical significance was performed using one-way ANOVA. *p < 0.05 vs Control, #p < 0.05 vs CIS.

Article Snippet: Currently, there is an increasing optimism of nanomedicine as it Abbreviations: A549 cell line, The human squamous cell lung cancer cell line; AO, acridine orange; ATCC, American type culture collection; Bax, BCL2-Associated X Protein; Bcl2, B-cell lymphoma protein 2; Beclin-1, BECN1 gene encoded protein; involved in autophagy, apoptosis and host defense; C13 NMR, Carbon-13 nuclear magnetic resonance; CIS, Cisplatin (positive control); CT, Understanding cycle threshold; DDS, drug-delivery systems; Delta Ct, corresponds to the difference between CtSOI and Ct of reference sequence (RS); EE, Entrapment efficiency; FT-IR analysis, Fourier-transform infrared spectroscopy; G0/G1 cell cycle arrest, resting phase is a period in the cell cycle/the first growth period of the cell cycle during interphase; G2/M phase, The arrest in G2 is mediated by the inhibition of M-phase promoting factor (MPF); GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; GC–MS, Gas chromatography–mass spectrometry; GMS, glyceryl monostearate; H460 cell line, large cell lung cancer cell line; HPGPC, high-performance gel permeation chromatography; HPLC, high-performance liquid chromatography; Mn, The number average molecular weight; MTT assay, colorimetric assay for assessing cell metabolic activity; NFI, net fluorescent intensities; NSCLC, non-small lung cancer; nTOPL, nano- Taraxacum officinale polysaccharide; one-way ANOVA, Analysis of variance; PDI,PI, polydispersity index; qRT-PCR, Real-Time Quantitative Reverse Transcription PCR; S-phase, synthesis phase of the cell cycle in which DNA is replicated; SLNs, solid lipid nanoparticles; TEM, Transmission electron microscopy; TOPL, Taraxacum officinale leaves derived polysaccharides; UV spectrum, Ultraviolet spectrum; WI-38 cell line, normal human lung cell line; ZP, zeta potential.

Techniques: Expressing, Standard Deviation, Control

Journal: Journal of Functional Foods

Article Title: Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma

doi: 10.1016/j.jff.2021.104781

Figure Lengend Snippet: Fig. 6. Autophagic cell death evaluation. A: Representative histograms for quantifying autophagy by flow cytometry using AO stain. B: Folds of autophagy increase in relation to control in A549 and H460 cell-lines. C: The level of mRNA Beclin-1 expression in A549 and H460 cell lines. Data are expressed as mean ± standard deviation for triplicates within an individual experiment. Statistical significance was performed using one-way ANOVA. *p < 0.05 vs Control, #p < 0.05 vs CIS.

Article Snippet: Currently, there is an increasing optimism of nanomedicine as it Abbreviations: A549 cell line, The human squamous cell lung cancer cell line; AO, acridine orange; ATCC, American type culture collection; Bax, BCL2-Associated X Protein; Bcl2, B-cell lymphoma protein 2; Beclin-1, BECN1 gene encoded protein; involved in autophagy, apoptosis and host defense; C13 NMR, Carbon-13 nuclear magnetic resonance; CIS, Cisplatin (positive control); CT, Understanding cycle threshold; DDS, drug-delivery systems; Delta Ct, corresponds to the difference between CtSOI and Ct of reference sequence (RS); EE, Entrapment efficiency; FT-IR analysis, Fourier-transform infrared spectroscopy; G0/G1 cell cycle arrest, resting phase is a period in the cell cycle/the first growth period of the cell cycle during interphase; G2/M phase, The arrest in G2 is mediated by the inhibition of M-phase promoting factor (MPF); GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; GC–MS, Gas chromatography–mass spectrometry; GMS, glyceryl monostearate; H460 cell line, large cell lung cancer cell line; HPGPC, high-performance gel permeation chromatography; HPLC, high-performance liquid chromatography; Mn, The number average molecular weight; MTT assay, colorimetric assay for assessing cell metabolic activity; NFI, net fluorescent intensities; NSCLC, non-small lung cancer; nTOPL, nano- Taraxacum officinale polysaccharide; one-way ANOVA, Analysis of variance; PDI,PI, polydispersity index; qRT-PCR, Real-Time Quantitative Reverse Transcription PCR; S-phase, synthesis phase of the cell cycle in which DNA is replicated; SLNs, solid lipid nanoparticles; TEM, Transmission electron microscopy; TOPL, Taraxacum officinale leaves derived polysaccharides; UV spectrum, Ultraviolet spectrum; WI-38 cell line, normal human lung cell line; ZP, zeta potential.

Techniques: Flow Cytometry, Staining, Control, Expressing, Standard Deviation